Review



prism 7900ht sequence detection system  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Thermo Fisher prism 7900ht sequence detection system
    Prism 7900ht Sequence Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prism+7900ht+sequence+detection+system/7500+fast+real+time+pcr+system/pm40583111-100-12-17
    Average 90 stars, based on 1 article reviews
    prism 7900ht sequence detection system - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: Combined ADAMTS10 and ADAMTS17 inactivation exacerbates bone shortening and compromises extracellular matrix formation
    Article Snippet: For each reaction, 2 μl cDNA, 0.5 μl of forward and reverse primers (100 μM stock) and SYBR Green PCR Master Mix (Applied Biosystems) were combined in a total reaction volume of 10 μl. .. The amplification and detection were performed on an ABI PRISM 7900HT Sequence Detection System (Applied Biosystems). ..

    Sequencing:

    Article Title: Combined ADAMTS10 and ADAMTS17 inactivation exacerbates bone shortening and compromises extracellular matrix formation
    Article Snippet: For each reaction, 2 μl cDNA, 0.5 μl of forward and reverse primers (100 μM stock) and SYBR Green PCR Master Mix (Applied Biosystems) were combined in a total reaction volume of 10 μl. .. The amplification and detection were performed on an ABI PRISM 7900HT Sequence Detection System (Applied Biosystems). ..

    Article Title: Inhibition of ATP-citrate lyase by bempedoic acid protects against abdominal aortic aneurysm formation in mice.
    Article Snippet: Abdominal aortic aneurysm (AAA) is a prevalent degenerative disease characterized by an exacerbated inflammation and destructive vascular remodeling.. Unfortunately, effective pharmacological tools for the treatment of this disease remain a challenge.. ATP-citrate lyase (ACLY), the primary enzyme responsible for acetyl-CoA biosynthesis, is a key regulator of inflammatory signaling in macrophages and lymphocytes.

    Article Title: Melatonin induces urethral contraction and increases intraurethral pressure via MT 1 receptor activation in female rats.
    Article Snippet: Melatonin is released by the pineal gland and exerts numerous physiological effects via specific G proteincoupled receptors named MT1 and MT2 receptors.. It is well known that melatonin acts as a key regulator of circadian rhythm through the activation of these receptors on the central nervous system.. However, the expression and function of melatonin receptors in the lower urinary tract remain unclear.

    Article Title: Lipid-associated macrophages are more abundant in subcutaneous than visceral adipose tissue in patients with obesity.
    Article Snippet: .. Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was carried out in an ABI PRISM 7900HT Sequence Detection System (ThermoFisher Scientific) using the following conditions: 2 min at 50 C, 10 min at 95 C followed by 40 cycles of 15 s at 95 C, and 1 min at 60 C. Relative mRNA levels were determined using the 2 ΔΔCt method and expressed as fold change in arbitrary units. ..

    Article Title: Influence of Dietary Fiber and Polyphenols During Pre-Gestation, Gestation, or Lactation on Intestinal Gene Expression.
    Article Snippet: Selected targets were measured using specific TaqMan® primers and probes for PCR (Applied Biosystems, AB, Weiterstadt, Germany): Fam117a (Rn01304319_m1), Ets1 (Rn01524757_m1), Hmox1 (Rn00561387_m1), Apln (Rn00581093_m1), Il17 (Rn01757168_m1), Fasn (Rn00569117_m1), and Lsd1 (Rn01181029_m1). .. Quantitative Real-Time PCR assays were performed in duplicate for each sample using the ABI PRISM 7900HT Sequence Detection System (Applied Biosystems). .. Gene expression levels were normalized to the housekeeping gene Gusb (Rn00566655_m1).

    Article Title: CD36 enrichment in HER2-positive mesenchymal stem cells drives therapy refractoriness in breast cancer
    Article Snippet: .. Total RNA from the tested cells was extracted using QIAzol (QIAGEN) according to the manufacturer’s instructions and as reported previously [ ], and RNA purity was verified by measuring the 260/280 absorbance ratio. cDNA was reverse transcribed from 1 μg of total RNA in a 20 μl reaction volume with SuperScript III (Invitrogen, Waltham, MA, USA) using oligo(dT) primers, and expression was measured by qRT‒PCR using an Applied Biosystems SYBR® Green dye-based PCR assay in an ABI Prism 7900HT sequence detection system (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: PR+ progenitors contribute to all mammary epithelial lineages
    Article Snippet: For epithelial subpopulations with a limited number of cells resulting in low levels of RNA, the first strand was amplified using the Advantage2 PCR kit (Clontech) and the optimal number of amplification cycles for each sample was individually determined by analyzing amplified cDNA aliquots on an ethidium bromide-stained 1.2% agarose gel. .. Relative quantification of Real-Time PCR (Ct) was performed on 4 ng of cDNA using an ABI PRISM 7900HT Sequence Detection System and Taqman gene expression assay mixes containing unlabelled PCR primers and FAM labeled Taqman MBG probes (Table 1-1). ..

    other:

    Article Title: Exploration of virulence and immune evasion functions of the candidate vaccine antigen SpyAD in the globally disseminated M1T1 group A Streptococcus strain.
    Article Snippet: RNA was extracted from 10 mL mid-logarithmic phase bacteria using a DNA/RNA Co-Extraction kit (Zymo Research #R2002).

    Quantitative RT-PCR:

    Article Title: Melatonin induces urethral contraction and increases intraurethral pressure via MT 1 receptor activation in female rats.
    Article Snippet: Melatonin is released by the pineal gland and exerts numerous physiological effects via specific G proteincoupled receptors named MT1 and MT2 receptors.. It is well known that melatonin acts as a key regulator of circadian rhythm through the activation of these receptors on the central nervous system.. However, the expression and function of melatonin receptors in the lower urinary tract remain unclear.

    Real-time Polymerase Chain Reaction:

    Article Title: Influence of Dietary Fiber and Polyphenols During Pre-Gestation, Gestation, or Lactation on Intestinal Gene Expression.
    Article Snippet: Selected targets were measured using specific TaqMan® primers and probes for PCR (Applied Biosystems, AB, Weiterstadt, Germany): Fam117a (Rn01304319_m1), Ets1 (Rn01524757_m1), Hmox1 (Rn00561387_m1), Apln (Rn00581093_m1), Il17 (Rn01757168_m1), Fasn (Rn00569117_m1), and Lsd1 (Rn01181029_m1). .. Quantitative Real-Time PCR assays were performed in duplicate for each sample using the ABI PRISM 7900HT Sequence Detection System (Applied Biosystems). .. Gene expression levels were normalized to the housekeeping gene Gusb (Rn00566655_m1).

    Article Title: PR+ progenitors contribute to all mammary epithelial lineages
    Article Snippet: For epithelial subpopulations with a limited number of cells resulting in low levels of RNA, the first strand was amplified using the Advantage2 PCR kit (Clontech) and the optimal number of amplification cycles for each sample was individually determined by analyzing amplified cDNA aliquots on an ethidium bromide-stained 1.2% agarose gel. .. Relative quantification of Real-Time PCR (Ct) was performed on 4 ng of cDNA using an ABI PRISM 7900HT Sequence Detection System and Taqman gene expression assay mixes containing unlabelled PCR primers and FAM labeled Taqman MBG probes (Table 1-1). ..

    Reverse Transcription:

    Article Title: CD36 enrichment in HER2-positive mesenchymal stem cells drives therapy refractoriness in breast cancer
    Article Snippet: .. Total RNA from the tested cells was extracted using QIAzol (QIAGEN) according to the manufacturer’s instructions and as reported previously [ ], and RNA purity was verified by measuring the 260/280 absorbance ratio. cDNA was reverse transcribed from 1 μg of total RNA in a 20 μl reaction volume with SuperScript III (Invitrogen, Waltham, MA, USA) using oligo(dT) primers, and expression was measured by qRT‒PCR using an Applied Biosystems SYBR® Green dye-based PCR assay in an ABI Prism 7900HT sequence detection system (Applied Biosystems, Foster City, CA, USA). ..

    Expressing:

    Article Title: CD36 enrichment in HER2-positive mesenchymal stem cells drives therapy refractoriness in breast cancer
    Article Snippet: .. Total RNA from the tested cells was extracted using QIAzol (QIAGEN) according to the manufacturer’s instructions and as reported previously [ ], and RNA purity was verified by measuring the 260/280 absorbance ratio. cDNA was reverse transcribed from 1 μg of total RNA in a 20 μl reaction volume with SuperScript III (Invitrogen, Waltham, MA, USA) using oligo(dT) primers, and expression was measured by qRT‒PCR using an Applied Biosystems SYBR® Green dye-based PCR assay in an ABI Prism 7900HT sequence detection system (Applied Biosystems, Foster City, CA, USA). ..

    SYBR Green Assay:

    Article Title: CD36 enrichment in HER2-positive mesenchymal stem cells drives therapy refractoriness in breast cancer
    Article Snippet: .. Total RNA from the tested cells was extracted using QIAzol (QIAGEN) according to the manufacturer’s instructions and as reported previously [ ], and RNA purity was verified by measuring the 260/280 absorbance ratio. cDNA was reverse transcribed from 1 μg of total RNA in a 20 μl reaction volume with SuperScript III (Invitrogen, Waltham, MA, USA) using oligo(dT) primers, and expression was measured by qRT‒PCR using an Applied Biosystems SYBR® Green dye-based PCR assay in an ABI Prism 7900HT sequence detection system (Applied Biosystems, Foster City, CA, USA). ..

    Polymerase Chain Reaction:

    Article Title: CD36 enrichment in HER2-positive mesenchymal stem cells drives therapy refractoriness in breast cancer
    Article Snippet: .. Total RNA from the tested cells was extracted using QIAzol (QIAGEN) according to the manufacturer’s instructions and as reported previously [ ], and RNA purity was verified by measuring the 260/280 absorbance ratio. cDNA was reverse transcribed from 1 μg of total RNA in a 20 μl reaction volume with SuperScript III (Invitrogen, Waltham, MA, USA) using oligo(dT) primers, and expression was measured by qRT‒PCR using an Applied Biosystems SYBR® Green dye-based PCR assay in an ABI Prism 7900HT sequence detection system (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: PR+ progenitors contribute to all mammary epithelial lineages
    Article Snippet: For epithelial subpopulations with a limited number of cells resulting in low levels of RNA, the first strand was amplified using the Advantage2 PCR kit (Clontech) and the optimal number of amplification cycles for each sample was individually determined by analyzing amplified cDNA aliquots on an ethidium bromide-stained 1.2% agarose gel. .. Relative quantification of Real-Time PCR (Ct) was performed on 4 ng of cDNA using an ABI PRISM 7900HT Sequence Detection System and Taqman gene expression assay mixes containing unlabelled PCR primers and FAM labeled Taqman MBG probes (Table 1-1). ..

    Quantitative Proteomics:

    Article Title: PR+ progenitors contribute to all mammary epithelial lineages
    Article Snippet: For epithelial subpopulations with a limited number of cells resulting in low levels of RNA, the first strand was amplified using the Advantage2 PCR kit (Clontech) and the optimal number of amplification cycles for each sample was individually determined by analyzing amplified cDNA aliquots on an ethidium bromide-stained 1.2% agarose gel. .. Relative quantification of Real-Time PCR (Ct) was performed on 4 ng of cDNA using an ABI PRISM 7900HT Sequence Detection System and Taqman gene expression assay mixes containing unlabelled PCR primers and FAM labeled Taqman MBG probes (Table 1-1). ..

    Gene Expression:

    Article Title: PR+ progenitors contribute to all mammary epithelial lineages
    Article Snippet: For epithelial subpopulations with a limited number of cells resulting in low levels of RNA, the first strand was amplified using the Advantage2 PCR kit (Clontech) and the optimal number of amplification cycles for each sample was individually determined by analyzing amplified cDNA aliquots on an ethidium bromide-stained 1.2% agarose gel. .. Relative quantification of Real-Time PCR (Ct) was performed on 4 ng of cDNA using an ABI PRISM 7900HT Sequence Detection System and Taqman gene expression assay mixes containing unlabelled PCR primers and FAM labeled Taqman MBG probes (Table 1-1). ..

    Labeling:

    Article Title: PR+ progenitors contribute to all mammary epithelial lineages
    Article Snippet: For epithelial subpopulations with a limited number of cells resulting in low levels of RNA, the first strand was amplified using the Advantage2 PCR kit (Clontech) and the optimal number of amplification cycles for each sample was individually determined by analyzing amplified cDNA aliquots on an ethidium bromide-stained 1.2% agarose gel. .. Relative quantification of Real-Time PCR (Ct) was performed on 4 ng of cDNA using an ABI PRISM 7900HT Sequence Detection System and Taqman gene expression assay mixes containing unlabelled PCR primers and FAM labeled Taqman MBG probes (Table 1-1). ..



    Similar Products

    90
    Thermo Fisher prism 7900ht sequence detection system
    Prism 7900ht Sequence Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prism+7900ht+sequence+detection+system/7500+fast+real+time+pcr+system/pm40583111-100-12-17
    Average 90 stars, based on 1 article reviews
    prism 7900ht sequence detection system - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher prism 7900ht pcr sequence detection system
    Prism 7900ht Pcr Sequence Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prism+7900ht+sequence+detection+system/7900ht+fast+real+time+pcr+system/pm40513767-88-7-14
    Average 90 stars, based on 1 article reviews
    prism 7900ht pcr sequence detection system - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher abi-prism 7900ht sequence detection system
    Abi Prism 7900ht Sequence Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prism+7900ht+sequence+detection+system/pm39972499-91-11-11
    Average 90 stars, based on 1 article reviews
    abi-prism 7900ht sequence detection system - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher -prism 7900ht sequence detection system
    Prism 7900ht Sequence Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prism+7900ht+sequence+detection+system/10__1165_slash_rcmb__2024___0238oc-551-14-19
    Average 90 stars, based on 1 article reviews
    -prism 7900ht sequence detection system - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results